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Image Search Results
Journal: Cell
Article Title: Thyroid hormone remodels cortex to coordinate body-wide metabolism and exploration
doi: 10.1016/j.cell.2024.07.041
Figure Lengend Snippet:
Article Snippet:
Techniques: Virus, Plasmid Preparation, Recombinant, Control, Protease Inhibitor, RNAscope, Multiplex Assay, Negative Control, Software, Modification
Journal: bioRxiv
Article Title: N-Ethylmaleimide-Sensitive Factor Deletion in Dopamine D2 Receptor Cells and Associated Neuronal and Behavioural Changes in Mice
doi: 10.64898/2025.12.17.694809
Figure Lengend Snippet: (a) Schematic illustration of the adeno-associated virus (AAV) constructs: AAV5-hSyn-EGFP, in which the human synapsin promoter (hSyn) drives EGFP expression, and AAV5-hSyn-DIO-mCherry, a Cre-dependent construct expressing mCherry. AAVs were injected into the striatum at 4 weeks of age and analysed at 5 weeks. (b) Representative images of the striatum showing EGFP and mCherry fluorescence in Nsf +/+ ;D2R-Cre, Nsf f/f , Nsf f/+ ;D2R-Cre, and Nsf f/f ;D2R-Cre mice. Scale bars=50 μm. (c) Quantification of mCherry-expressing cells relative to EGFP-expressing cells in the striatum ( Nsf f/f mice: n=3; Nsf f/f ;D2R-Cre mice: n=4; Nsf f/+ ;D2R-Cre mice: n=5; Nsf f/+ mice: n=7). (d) Representative coronal brain sections through the anterior commissure (0 axial level) from 3-week-old Nsf f/f (upper panel) and Nsf f/f ;D2R-Cre (lower panel) mice. (e) Average striatal area normalised to total brain area in Nsf f/f and Nsf f/f ;D2R-Cre mice (n=8 per group). Scale bars=500 μm. (f) Total striatal area relative to total brain area in Nsf f/f and Nsf f/f ;D2R-Cre mice (n=6 per genotype). (g) Absolute striatal area (n=6 per genotype). (h) Total brain area excluding the striatum in Nsf f/f and Nsf f/f ;D2R-Cre mice (n=6 per genotype). (i) Brain weight of Nsf f/f and Nsf f/f ;D2R-Cre mice (n=8 per genotype). Data are presented as mean±SEM. Student’s t -test (c, f-i) and two-way repeated measures ANOVA, F(1,20)=2.663, p =0.001 (e); ** p <0.01 (c, f, g, i).
Article Snippet: A mixture of viral vectors,
Techniques: Virus, Construct, Expressing, Injection, Fluorescence